| Dissertation |
Thesis (M.Sc.) --NUI, 2011 at Department Anatomy, UCC. |
| Summary |
Neuropeptide Y (NPY) is a regulatory peptide involved in a number of key physiological functions, including stimulation of food and water intake and blood pressure regulation. NPY is also thought to be involved in suppressing epileptic seizures by inhibiting glutamatergic synaptic transmission. In this project, the main concern was with the effect of valproic acid, 12-0-tetracanoylphorbol-13-acetate (TPA) and amitriptyline treatment on mRNA expression of NPY in SH-SY5Y cells. Valproic acid (VPA) is an antiepileptic and antidepressant drug, which is thought to interact with the extracellular signal-related kinase pathway to increase NPY expression, resulting in its antiepileptic effect. TPA, a phorbol ester, is known to increase activation of NPY by activating protein kinase C and, in turn, extracellular signal-related kinase 1 and 2. Amitriptyline is a tri-cyclic antidepressant, it has been shown to promote tropomyosin receptor kinase A and B dimerization. As a result, amitriptyline increases AP-1 DNA binding, which can lead to up-regulation of NPY expression. The mRNA expression levels of NPY were analysed using quantitative PCR. The results showed that both VPA and TPA increased expression of NPY significantly, while amitriptyline increased expression significantly when compared to TPA. |
| Subject |
Valproic acid -- Therapeutic use.
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Nervous system -- Drug Therapy -- Diseases.
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| Collection |
Theses Masters
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Theses Anatomy Department
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| Description |
97 p. : ill. ; 30 cm. |
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